The Phylum Nematoda

 

Etymology:- From the Greek Nema for Thread and Eidos for form.

 

Characteristics of Nematoda:-

1)Bilaterally symmetrical, and vermiform.

2)Body has more than two cell layers, tissues and organs.

3)Body cavity is a pseudocoel, body fluid under high pressure.

4)Body possesses a through gut with a subterminal anus.

5)Body covered in a complex cuticle.

6)Has a nervous system with pharyngeal nerve ring.

7)Has no circulatory system (no blood system)

8)Reproduction normally sexual and gonochoristic.

9)Feed on just about everything.

10)Live just about everywhere, many species are endoparasites.

 

     Nematodes are the most speciose phylum after the arthropods, they occur in nearly every habitat including as parasites in all

sorts of plants and animals, (they don't like dry places however). One species is known that can live in old vinegar (Turbatrix

aceti)and another that as only been found in German beer mats. Though only about 80 000 species have been described some

scientists estimate there may be as many as a million species all told. They can occur in very dense numbers in the soil and rotting

vegetation, as many as 90 000 have been found in a single rotting apple, while millions occur in the top 3cm (1 inch) of a square

metre of good quality soil. While there are a huge number of free living Nematodes there are also a large number of parasitic

species, many of which cause diseases to man and other animals as well as to plants, nearly every living organism has been found

to be parasitised by one species of nematode or another. Most nematodes are reasonably small, they range in size from 100

micrometres in length (1/10th of a mm or 1/250th of an in) to the female Giant Nematode Dioctophyme renale which may be up

to 1 metre, or 3 ft long

 

     Free living nematodes are long thin worms with transparent and typically curled bodies, parasitic species have a variety of

less streamline shapes relating to their degenerate parasitic life styles, one unifying characteristic that makes the phylum unique is

the lack of cilia or flagella, even the sperm of nematodes are amoeboid. Nematodes as parasites have been known for a long time

and the earliest recorded literary mention of them is an Egyptian papyrus from 1500 BC, they are also mentioned by the ancient

Greeks Aristotle and Hippocratis the father of scientific medicine.

 

Ecology

 

     Nematodes live in a vast variety of habitats, ecologically they can be divided into free living forms and parasitic forms. Free

living forms have a simple life cycle involving 4 juvenile instars on the path from egg to adult. Parasitic species have developed a

wide range of variations on this basic theme. The variations involve whether there is a secondary host and the amount of time

spent in one or either hosts. There is also considerable variability in the way that they move from one host species to another. thus

while many species lay eggs that pass out of the primary host with the feces where they are eaten by the secondary host which

then gets eaten in turn by the primary host after the Nematodes have developed. Because it is not always totally reliable that the

secondary host will be eaten just as the Nematode larvae have developed into the infective stage many species have the ability to

encyst themselves in the muscle or cuticle of their secondary hosts.

 

     Some species use another animal to transport them from one host to another thus Wuchereria bancrofti releases minute

live young called 'microfilaria' into the primary hosts blood stream rather than eggs into the digestive tract. These microfilaria get

ingested by mosquitoes when they feed on an infected person. Inside the mosquito they live in the mosquitoes gut where they

develop until the Larva 3 stage wait for the mosquito to bite another host whereupon they enter the host via the mosquitoes

proboscis sheath and the wound it makes in the hosts skin.

 

Nematodes in Mankind

 

Human beings, along with all other living things are host to numerous Nematode parasites. The commonest of these is Ascaris

lumbricoides with an estimated 700 million people effected globally, this Nematode is not normally fatal and in low numbers may

have very little effect on adults, however in heavy doses it can be quite debilitating, especially for children. The Nematodes

infecting mankind include several species of filarial worms, the most important of these are Wuchereria bancrofti and Brugia

malayi which are very similar and cause lymphatic filariasis, Onchocerca volvulus which causes River Blindness and Loa loa

which causes Loiasis. Other species are Dranunculus medinensis known as Guinea Worm, Trichinella spiralis causing

Trichinosis, Necator americanus and Ancylostoma duodenale causing Hookworm, Enterobius vermicularis causing Pinworms

and Trichuris trichuria causing Whipworm or Trichuriasis.

Anatomy

 

     Basically a Nematode is a long hollow tube within which is another tube, the alimentary canal and the reproductive organs.

Nematodes are round in cross section, this is because unlike the other worms that below them in the phyla table they maintain their

body fluids under great pressure (on average internal pressure in a nematode equals 70mm of mercury or 1.49 PSI, with a

maximum recorded value of 125mm of mercury or 2.41 PSI). To contain this high pressure nematodes have an extremely tough,

yet elastic and flexible cuticle. This cuticle consists of up to 9 layers of proteinaceous fibres, with 3 layers being easily discerned,

these are called, from the outside in, the cortex, the matrix layer and the fibre layer. Despite its complexity the Nematode cuticle is

permeable to both water and gases, so respiration occurs through it. Beneath the cuticle is a hypodermis and a layer of longitudinal

muscle. The combination of the flexure of these muscles with the high pressure of the system produces a characteristic whip-like

wriggle that Nematodes use to swim. Scientifically this is called undulatory propulsion with sinusoidal waves passing back along

the body.

 

     At the anterior (head) end there is a mouth which has 3 lips behind which predatory species possess a few teeth, this leads

to a pharynx which is triangular in cross section. Because of the high pressure within the body unsupported organs such as the

intestines tend to collapse in much the same way that an uninflated bicycle tube tends to become oval or flat in cross section when

laid flat on the table. The pharynx of Nematodes is an efficient pump and forces food into the intestines, there is a one way valve

between the intestines and the pharynx. The pharynx can, when this valve is closed, be used to suck liquid food into the mouth.

Digestion is rapid and feces are expelled under pressure. This pressure is so great that the parasitic nematode Ascaris

lumbricoides which is about 12cm to 18cm long (5 to 7 inches) may shoot its feces 60cm or 2 feet into the air.

 

     Nematodes, especially free living forms generally have a reasonably well developed nervous system. This is comprised of a

circum-pharyngeal nerve ring made up from 4 nerve ganglia from which 6 longitudinal nerves extend down through the body to the

various parts of the gut and the reproductive organs. There are also 6 shorter nerves which extend forwards from the

circum-pharyngeal ganglia towards the mouth. Nematodes have no circulatory or respiratory organs and the excretion of

metabolic waste is via two simple ducts or tubules which have no nephridia or flame cells.

 

     Nematodes are copiously reproductive and most of their body cavity, which is a pseudocoelom is filled with paired sets of

reproductive organs, either ovaries or testes. Males and females copulate and the male introduces sperm to the females vagina

with the help of 2 stiff horny spicules that are a part of his cloaca. Fertilisation is internal and females lay eggs over a prolonged

time period, thus a female Ascaris lumbricoides may lay her eggs at the rate of 200,000 per day and have had a total 27 million

eggs within her at the start of her reproductive career. Young nematodes hatch from these eggs and go through 4 moults before

they become adults.

 

Classification of the Nematoda

     Phylum

                Class

                            Order

     Nematoda

                Adenophorea

                            Enoplida

                            Isolaimida

                            Mononchida

                            Dorylaimida

                            Trichocephalida

                            Mermithida

                            Muspiceida

                            Araeolaimida

                            Chromadorida

                            Desmoscolecida

                            Desmodorida

                            Monhysterida

                Secernentea

                            Rhabdita

                            Strongylida

                            Ascaridida

                            Spirurida

                            Camallanida

                            Diplogasterida

                            Tylenchida

     Below are two lists of of organisms and the Nematodes that infect them, this is only a minute example covering only two

groups of organisms Commercial Plants and Domestic Animals, in the plant list many of the species listed for one plant group also

infest other plant groups.

 

Some Nematode Parasites of

Domestic Animals

     Animal Group

                  Nematode Species

     Rodents

                  Angiostrongylus cantonensis

                  Nippostrongylus brasiliensis

                  Syphacia obvelata

                  Capillaria hepatica

     Cattle

                  Dictyocaulus viviparus

                  Oesophagostomum radiatum

                  Onchocerca gutterosa

     Horses

                  Strongylus edentatus

                  Parascaris equorum

                  Oxyuris equi

     Pigs

                  Stephanurus dentatus

                  Ascaris suum

     Sheep

                  Haemonchus contortus

                  Ostertagia ostertagi

     Dogs

                  Driofillaria spp.

                  Dioctophyma renale

     Chickens

                  Syngamus trachea

                  Ascaridia galli

                  Heterakis gallinarum

 

 

Some Nematode Parasites of

Commercial Plants

     Plant Group

                    Nematode Species

     Chrysanthemums

                    Aphelenchoides ritzemabosi

     Buddleja

                    Aphelenchoides ritzemabosi

     Scabious

                    Aphelenchoides blastophthorus

     Lillies

                    Aphelenchoides fragaria

     Ferns

                    Aphelenchoides fragaria

     Cacti

                    Heterodera cacti

     Wallflowers

                    Heterodera cruciferae

     Figs

                    Heterodera fici

     Hops

                    Heterodera humuli

     Roses

                    Xiphinema spp.

     Narcissus

                    Pratylenchus penetrans

     Potatoes

                    Ditylenchus destructor

     Pot Plants

                    Meloidogyne javanica

     Herbaceous Plants

                    Ditylenchus dipsaci

     Many plants

                    Longidorus elongatus

     Many Plants

                    Longidorus Macrosoma